Q. During cDNA formation, what would happen if DNA formed by reverse transcriptase is not treated with the alkali?
(a) cDNA will not be digested
(b) mRNA will not be digested
(c) Hydrogen bonds will not form between base pairs
(d) mRNA will not be formed.
Answer (b) : The cDNA formation involves the alkaline denaturation of the mRNA-cDNA hybrid. The double stranded DNA molecule formed after the activity of reverse transcriptase is treated with alkali to digest mRNA.
