Explain the importance of
(i) ori, (ii) ampR and (iii) rop in the E.coli vector shown below.
Answer (i) ori : ori is the origin of replication. This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within host cells. It controls the copy number of the linked DNA.
(ii) ampR : Gene for ampicillin resistance which helps in selecting the transformants.
(iii) rop : rop codes for the proteins involved in the replication of the plasmid.
