Rearrange the following in the correct sequence to accomplish an important biotechnological reaction :
(i) Denaturation of ds-DNA
(ii) Chemically synthesised oligonucleotides
(iii) Primers
(iv) Complementary region of DNA
(v) Thermostable DNA polymerase (from Thermus aquaticus)
(vi) Nucleotides provided
(vii) Genomic DNA template
(viii) In vitro synthesis of copies of DNA of interest
(ix) Enzyme DNA-polymerase.
Answer. The correct sequence to accomplish biotechnological reaction is :
Denaturation of ds DNA
↓
Genomic DNA template
↓
Chemically synthesised oligonucleotides (Primers)
↓
Complementary regions of DNA
↓
Thermostable DNA polymerase (Enzyme polymerase) (from Thermus aquaticus)
↓
dNTPs (Nucleotides) provided
↓
In vitro synthesis of copies of DNA of interest
