(a) What is EcoRI? What does ‘R’ represent in this?
(b) Give the palindromic nucleotide sequence recognised by it.
(c) Explain its action.
Answer (a) Enzyme EcoRI is named as follows :
The capital letter E comes from the genus Escherichia. The letters co are derived from the species name coli. The letter R is from RYI3 (strain). The Roman number I indicates that itwas the first enzyme isolated from the bacterium E. coli RYI3.
(b) EcoRI is a restriction endonuclease enzyme it recognises
base sequences
5′-GAATTC-3′
3′-CTTAAG-5′ in DNA duplex.
(c) It cut each of the two strands between G and A producing sticky ends.