Q. Which of the following statements regarding PCR is correct?
(a) Taq polymerase, which is isolated from bacterium Thermus aquaticus is stable at low temperature only.
(b) With the help of DNA ligase, the complementary sticky ends of the DNA are joined to produce a rDNA.
(c) Since the sequence of primers are complementary to 5′ end of the template DNA, they anneal to it.
(d) DNA purified from the cell is precipitated by adding hot ethanol.
Answer (b) : Taq polymerase isolated from bacterium Thermus aquaticus is stable at high temperature. Sequence of primers are complementary to 3′ end of the template. Purified DNA is precipitated by adding chilled ethanol.
