Write the role of ‘ori’ and ‘restriction’ site in a cloning vector pBR322.
Answer Origin of replication (ori) site in cloning vector pBR322 is a sequence from where replication starts. Any piece of DNA when linked to this sequence can be made to replicate within host cell. Restriction site within the markers tetR and ampR genes permit an easy selection for cells transformed or the recombinant pBR322.
