Identification of stages of gamete development, i.e., T.S. of testis and T.S. of ovary through permanent slides (from grasshopper/mice) class12 biology practical

This Class 12 Biology practical focuses on identifying different stages of gamete development using permanent slides of T.S. of testis and T.S. of ovary from grasshopper or mice. This activity helps students understand the process of gametogenesis, i.e., formation of sperm and ovary.

In the T.S. of testis, students observe seminiferous tubules containing spermatogonia, primary and secondary spermatocytes, spermatids, and mature spermatozoa. The presence of Sertoli cells and interstitial spaces adds to structural clarity.

In the T.S. of ovary, learners identify primary follicles, secondary follicles, Graafian follicle, and the developing ovum. The surrounding follicular cells and stroma highlight the sequential stages of oogenesis.

This practical is important for CBSE Class 12 examinations because it helps students visualize reproductive biology concepts. Drawing neat diagrams and labeling stages accurately improves practical record work and enhances understanding for viva questions and board exam preparation. 

Aim: Identification of stages of gamete development, i.e., T.S. of testis and T.S. of ovary through permanent slides (from grasshopper/mice).

Principle: In all male and female organisms gamete formation takes place in their gonads, i.e., testis and ovary respectively. The process of gamete formation, called gametogenesis involves meiotic cell division. The gametogenic development in testis is called spermatogenesis and in ovary it is oogenesis. They exhibit marked differences and can be examined in transverse section (T.S.) of these organs.

Requirement: Permanent slides of T.S. of testis and ovary, compound microscope,

lens-cleaning paper and cleaning fluid

Procedure

(i) Clean the slide and microscope’s eye and objective lenses with the help of lens cleaning paper using any cleaning fluid.

(ii) Place the slide on the stage of the microscope and observe first under lower magnification and then in higher magnification. Observe various stages of gamete development.

(iii) Record your observations in the notebook and draw labelled diagrams.

Observation

T.S. of testis

(i) You will observe a large number of seminiferous tubules under lower magnification. Observe a complete tubule in higher magnification and view various stages of gamete development from periphery towards lumen (Fig. 4.1) and identify the following types of cells namely, Germinal epithelium, Spermatogonial cells, Primary spermatocytes, Secondary spermatocytes, Spermatids and Spermatozoa.

(ii) In T.S. of testis the space between tubules are filled with blood vessels and a specific cell type called Leydig's cell or Interstitial cells.

T.S. of Ovary

(i) In the section of ovary, there is a mass of tissue lined with germinal epithelium. Inside that you will observe an ovum, which is a cell surrounded by one to several layers of follicular cells. As the ovum matures, the number of surrounding follicular cell layer increases (Fig. )

(ii) In the later stage of follicular development a cavity called antrum appears.

(iii) The cavity gets further enlarged and the follicle grows bigger. This is the stage of Graafian follicle ready to release the ovum (ovulation).

(iv) In the next stage, you may notice a Corpus luteum, and/or Corpus albicans, which differ from each other and also from Graafian follicle in their features.

Discussion

Spermatogenesis is a continuous process after attainment of puberty, and that is why gamete development and spermatozoa are observed in a single seminiferous tubule. In case of ovary, the follicular development stages are observed.

Precaution

• First observe the slide under low power and then under the high power of the microscope.
• Use fine adjustment of the microscope for focussing the slide under high power.