1. Restriction enzymes were discovered by
Smith and Nathans
Alexander Fleming
Berg
None
2. Bacteria protect themselves from viruses by fragmenting viral DNA with
Ligase
Endonuclease
Exonuclease
Gyrase
3. Klenow fragment is derived from
DNA Ligase
DNA Pol-I
DNA Pol-II
Reverse Transcriptase
4. Southern blotting is
Attachment of probes to DNA fragments
Transfer of DNA fragments from electrophoretic gel to nitrocellulose sheet
Comparison of DNA fragments to two sources
Transfer of DNA fragments to electrophoretic gel from cellulose membrane
5. ELISA is
Using radiolabelled second antibody
Usage of RBCs
Using compliment mediated cell lysis
Addition of substrate that is converted into a coloured end product
6. The Golden Rice variety is rich in
Vitamin C
Î’-carotene and ferritin
Biotin
Lysine
7. The DNA fragments have sticky ends due to
Endonuclease
Unpaired bases
Calcium ions
Free methylation
8. Plasmids are used as cloning vectors for which of the following reasons?
Can be multiplied in culture
Self-replication in bacterial cells
Can be multiplied in laboratories with the help of enzymes
Replicate freely outside bacterial cells
9. The human genome project was launched in the year
1980
1973
1990
1989
10. The vaccines prepared through recombinant DNA technology are
Third generation vaccines
First generation vaccines
Second generation vaccines
None
11. Which is a genetically modified crop?
Bt-cotton
Bt-brinjal
Golden rice
All
12. PCR technique was invented by
Karry Mullis
Boyer
Sanger
Cohn
13. The first transgenic plant to be produced is
Brinjal
Tobacco
Rice
Cotton
14. RNA interference helps in
Cell proliferation
Micropropagation
Cell defence
Cell differentiation
15. Which of the following is the quality of improved transgenic basmati rice?
Gives high yield but no characteristic aroma
Gives high yield and is rich in vitamin A
Does not require chemical fertilizers and growth hormones
Resistant to insects and diseases
16. The first clinical application of gene therapy over a 4 year old girl was for
Adenosine deaminase deficiency
Adenosine deficiency
Growth deficiency
Adenine deficiency
17. Excision and insertion of a gene is called
Biotechnology
Genetic engineering
Cytogenetics
Gene therapy
18. The expression of a transgene in the target tissue is identified by a
Transgene
Promoter
Enhancer
Reporter
19. ———– is used as a vector for cloning into higher organisms
Retrovirus
Baculovirus
Salmonella typhimurium
Rhizopus nigricans
20. Which bacterium is used in the production of insulin by genetic engineering?
Saccharomyces
Rhizobium
Escherichia
Mycobacterium
1- a
2- b
3- b
4- b
5- d
6- b
7- b
8- b
9- 3
10- a
11- d
12- a
13- b
14- c
15- b
16- a
17- b
18- d
19- a
20- c
Smith and Nathans
Alexander Fleming
Berg
None
2. Bacteria protect themselves from viruses by fragmenting viral DNA with
Ligase
Endonuclease
Exonuclease
Gyrase
3. Klenow fragment is derived from
DNA Ligase
DNA Pol-I
DNA Pol-II
Reverse Transcriptase
4. Southern blotting is
Attachment of probes to DNA fragments
Transfer of DNA fragments from electrophoretic gel to nitrocellulose sheet
Comparison of DNA fragments to two sources
Transfer of DNA fragments to electrophoretic gel from cellulose membrane
5. ELISA is
Using radiolabelled second antibody
Usage of RBCs
Using compliment mediated cell lysis
Addition of substrate that is converted into a coloured end product
6. The Golden Rice variety is rich in
Vitamin C
Î’-carotene and ferritin
Biotin
Lysine
7. The DNA fragments have sticky ends due to
Endonuclease
Unpaired bases
Calcium ions
Free methylation
8. Plasmids are used as cloning vectors for which of the following reasons?
Can be multiplied in culture
Self-replication in bacterial cells
Can be multiplied in laboratories with the help of enzymes
Replicate freely outside bacterial cells
9. The human genome project was launched in the year
1980
1973
1990
1989
10. The vaccines prepared through recombinant DNA technology are
Third generation vaccines
First generation vaccines
Second generation vaccines
None
11. Which is a genetically modified crop?
Bt-cotton
Bt-brinjal
Golden rice
All
12. PCR technique was invented by
Karry Mullis
Boyer
Sanger
Cohn
13. The first transgenic plant to be produced is
Brinjal
Tobacco
Rice
Cotton
14. RNA interference helps in
Cell proliferation
Micropropagation
Cell defence
Cell differentiation
15. Which of the following is the quality of improved transgenic basmati rice?
Gives high yield but no characteristic aroma
Gives high yield and is rich in vitamin A
Does not require chemical fertilizers and growth hormones
Resistant to insects and diseases
16. The first clinical application of gene therapy over a 4 year old girl was for
Adenosine deaminase deficiency
Adenosine deficiency
Growth deficiency
Adenine deficiency
17. Excision and insertion of a gene is called
Biotechnology
Genetic engineering
Cytogenetics
Gene therapy
18. The expression of a transgene in the target tissue is identified by a
Transgene
Promoter
Enhancer
Reporter
19. ———– is used as a vector for cloning into higher organisms
Retrovirus
Baculovirus
Salmonella typhimurium
Rhizopus nigricans
20. Which bacterium is used in the production of insulin by genetic engineering?
Saccharomyces
Rhizobium
Escherichia
Mycobacterium
1- a
2- b
3- b
4- b
5- d
6- b
7- b
8- b
9- 3
10- a
11- d
12- a
13- b
14- c
15- b
16- a
17- b
18- d
19- a
20- c
