Q. Which of the following is a restriction endonuclease?
(a) DNaseI (b) RNase (c) HindII (d) Protease
Answer (c) : HindII is the first restriction endonuclease. It was isolated from Haemophilus influenzae Rd. It always cut DNA at specific position producing blunt ends. DNase I is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide non-specially. RNase is a type of nuclease that catalyses the degradation of RNA into smaller components. It can be endoribonuclease or exoribonuclease. A protease is an enzyme that perform proteolysis, i.e., protein catabolism by hydrolysis of the peptide bonds.
