Q. Which of the following restriction enzymes produces blunt ends?
(a) SalI
(b) EcoRV
(c) XhoI
(d) HindIII
Ans. (b) : EcoRV is a type II restriction endonuclease isolated from certain strains of E.coli. It creates blunt ends. It recognises the palindromic sequence of 6 bases as shown here:
SalI, XhoI and HindIII restriction enzymes produce sticky ends.
